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The Group of Prof. Dr. Walter at the Centre of Optical Technologies (ZOT) aims at developing novel correlative microscopy solutions to facilitate imaging across scales in life sciences – in close collaborations with regional industrial partners - We plan to combine advanced fluores-cence microscopy with electron microscopy to visualize molecules within their subcellular con-text at high resolution using Correlative Light and Electron Microscopy (CLEM). Specifically, we envision a unique advanced super-resolution and volume CLEM workflow under cryo-conditions (Correlative Cryo-Super-Resolution Light and Focused Ion Beam Scanning Electron Microscopy) that overcomes the current limitations, pushes correlative microscopy further and allows us, together with our collaboration partners, to tackle specific biomedical research questions that could not be answered before due to the lack of accessible solutions. This is a highly interdisci-plinary project between physics, engineering, optics, life and computer sciences, and we are looking for a

A PhD Student or part-time Postdoc in Advanced Optical Microscopy Development (f/m/d)
- Part-Time 60% (23.7 hrs/week) –

to help us tackle several biomedical showcase projects (from nanoplastics to cell infections) by building and applying novel CLEM techniques.

Your future area of responsibility

For the implementation of the project, according to your skill set and interests, your main task is to (1) build a first cryo-super-resolution microscope (Structured Illumination Microscopy). You will also work on (2) establishing Focused Ion Beam Scanning Electron Microscopy (FIBSEM) for biological samples at Aalen University, and (3) setting up correlative (automated) workflows between the two imaging techniques.

Your primary responsibilities will include

  • Building a super-resolution microscope at room temperature, including hardware control and image analysis
  • Implementation at cryogenic conditions
  • Application to biomedical research questions
  • FIBSEM data acquisition at a few nanometers’ resolution
  • Correlative imaging using light & electron microscopy
  • Optimization of electron microscopy sample carriers by micropatterning
  • Close collaboration with Carl Zeiss GmbH & Leibniz Institute of Photonic Technology
  • Confocal microscopy
  • Sample preparation and optimization
  • Cell culture

        Your Profile:

        • Master’s degree in physics, biomedical engineering, or similar
        • Proven experience in advanced fluorescence microscopy and optical alignment
        • Proven experience in image processing and programming
        • Highly proactive, driven-, and an independent team player

                In addition, it would be beneficial to have experience in

                • Cell biology, cell culture, and tackling biomedical research using bioimaging
                • Hardware control
                • Electron microscopy
                • Cryo-microscopy
                • Correlative light and electron microscopy

                Our Offer:

                • A crisis-proof job
                • An exciting and varied field of activity
                • Cooperation in a friendly and motivated team
                • Flexible and family-friendly working hours
                • A company pension scheme (supplementary pension)
                • Attractive company health management offers
                • Modern office equipment
                • Regular opportunities for further training
                • A Job ticket from the state of Baden-Württemberg
                • Childcare spots in the Einsteinchen day care centre at Aalen University of Applied Sci-ences

                The position is limited to three years.

                        The position will be classified in pay group 13 of the collective agreement for the public service of the federal states (TV-L), provided that the legal and personal requirements are met. Severely disabled applicants who qualify will be given special consideration. Further information can be found at http://www.hs-aalen.de/sbv

                        Aalen University aims to increase its proportion of women and therefore expressly encourages qualified women to apply. For further information, please contact the Equal Opportunities Officer, Miss Sabine Gold https://www.hs-aalen.de/chancengleichheit

                        Ask Us!
                        Would you like to know more about this position? Please feel free to contact Professor Dr. Andreas Walter andreas.walter@hs-aalen.de

                        Are you interested?

                        If you are interested in R&D in advanced microscopy with different radiations (from light to elec-trons), biophotonics, biomedicine, and image & data processing and want to help push the fron-tiers in cell biology, we look forward to receiving your application (motivation letter, CV, 2 refer-ence letters or contact list of referees, and any other relevant information).
                        Then we look forward to receiving your detailed application (reference number 22-09-04) by 03.10.2022 via our online application portal.

                         

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